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  • 860336 - 3,3′,5,5′-Tetramethylbenzidine

Aldrich

3,3′,5,5′-Tetramethylbenzidine

860336 -

≥99%

DOWNLOAD MSDS (PDF)

Synonym: BM blue, Sure Blue TMB, TMB Blotting Plus, TMB substrate, TMB

  • CAS Number: 54827-17-7

  • Linear Formula: [-C6H2(CH3)2-4-NH2]2

  • Molecular Weight: 240.34

  • Beilstein Registry Number: 2808541

  • EC Number: 259-364-6

  • MDL number: MFCD00007748

  • PubChem Substance ID: 24888505

3,3′,5,5′-Tetramethylbenzidine ≥99%
#

Properties

Related CategoriesAmines, C11 to C38, Nitrogen Compounds
assay≥99%
mp168-171 °C(lit.)

Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8

Packaging

1, 5, 25 g in glass btl

100 mg in glass btl

Price and Availability

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Documents

Certificate of Analysis

Certificate of Origin

FT-IR Raman
FT-NMR
Structure Search
Similar Products

Safety Information

SymbolGHS07  GHS07
Signal wordWarning
Hazard statementsH315-H319-H335
Precautionary statementsP261-P305 + P351 + P338
Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard CodesXi
Risk Statements36/37/38
Safety Statements26-36
WGK Germany3
RTECSDV2300000
Technical information & documentation associated with this product is available in the Safety & Documentation tab.

References

1. Holland, V.R., et al. Tetrahedron 30, 3299, (1974)

2. D.D. Garner et al. J. Forensic Sci. 21, 819, (1976)

3. J.C. Standefer, D. Vanderjagt Clin. Chem. 23, 749, (1977)

4. R.C. Lijana, M.C. Williams J. Lab. Clin. Med. 94, 266, (1979)

5. Liem, H.H. et al. Anal. Biochem. 98, 388, (1979)

6. Bos, E.S., et al. J. Immunoassay 2, 187, (1981)

7. P.C. Andrews, N.I. Krinsky Anal. Biochem. 127, 346, (1982)

8. H. Gallati, I. Pracht J. Clin. Chem. Clin. Biochem. 23, 453, (1985)

Aldrich MSDS 1, 1679:B / Corp MSDS 1 (2), 3292:A / FT-IR 2 (2), 2090:C / FT-IR 1 (1), 1236:D / FT-NMR 1 (2), 529:B / FT-NMR 1 (1), 1236:D / IR-Spectra (3), 740:D / RegBook 1 (1), 1423:J / Sigma FT-IR 1 (2), 218:D / Stains and Dyes Ref, 693 / Structure Index 1, 222:D:3


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